Synthesis and characterization of silver nanoparticles using Datura metel L. (Solanaceae) leaf extract and its larvicidal activity on Epilachna vigintioctopunctata F.

Abstract

Insecticidal activities of synthesized silver nanoparticles of leaf extract of Datura metel L. (Solanaceae) (DM) against grubs Epilachna vigintioctopunctata F. (Coleoptera, Coccinellidae) at varying levels of concentrations was evaluated. DM leaf extract was used to create AgNPs, and nanoparticle production could be seen after six hours. UV-vis spectrophotometer, Particle size analyzer, FTIR and SEM analysis were used to confirm the synthesis of AgNPs. GCMS spectra of leaf extract of DM showed 20 substances, of which nine were known s phytochemicals and the others were unidentified. UV-visible spectra to analyse the Surface Plasmon Resonance for AgNPs revealed in the range of 366 - 374nm. LC50 values for the AgNPs synthesized leaf extracts calculated 24 h after treatment against the fourth instar larvae of E. vigintioctopunctata using probit analysis revealed the LC50 of the aqueous leaf extract as 252.31ppm and that of AgNPs synthesized leaf extract as 396.09ppm. When comparing the aqueous and AgNPs synthesized leaf extracts, AgNPs nanoparticles synthesized leaf extracts were more efficient as larvicide than aqueous leaf extract.